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Rabbit anti-GLP-1R, PKA, phospho-PKA (Thr197), CREB, phospho-CREB (Ser 133), tumor necrosis factor-alpha (TNF-), interleukin-1 beta (IL-1), and IL-6 primary antibodies were purchased from Abcam (Cambridge, UK)

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Researchers subsequently determined that MT-IIs effect on sexual arousal is mediated via interaction with the MC-3 and MC-4 receptors in the central nervous system, which function independently of the MC-1 receptors in melanocytes responsible for tanning [20]